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The oldest Mediterranean civilization, Greek, Roman or Egyptian have extensively used painting techniques based on mixtures of encaustic (probably rich in bee wax), mineral pigments (iron, copper, manganese oxides) and tempera. Vegetal oils, such as flax, walnut or poppyseed oil were known to ancient Egyptians, Greeks or Romans, but no precise indication of their use in painting may be found. Tempera is a fluid mixture of binder (organic medium), water and volatile additives (vegetal essential oils). Organic binders used by Italian artists were proteinaceous materials available from animal sources (whole egg, animal glues or milk). At the end of the roman empire and up to the Renaissance period (15th century), this ancient technique was lost and replaced by oil paint and/or tempera. In Italy and Greece, olive oil was used to prepare pigment mixtures but the drying time was excessively long and tedious in the case of figures. This drawback led a German monk, Theophilus, in the 12th century to warn against paint recipes including olive oil (Schoedula Diversarum Artium). It was reported that Aetius Amidenus, a medical writer in the 5th century, mentioned the use of a drying oil as a varnish on paintings. Similarly, it seems that perilla oil was used in Japan in painting after addition of lead in the 8th century. In the 14th century, Cennino Cennini presented a painting procedure integrating tempera painting covered by light oily layers.
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